Search for tag: "imaging"

01:06:14 Brooks Crickard Seminar Brooks Crickard Seminar	Brooks Crickard Seminar Zoom Recording ID: 698721325 UUID: erMYqwksSuSfocGtml45Ig== Meeting Time: 2020-04-07T13:46:52Z sequence alignment target recognition base pairs motor protein quick question search process double strand metal barriers d.n.a. molecule accessory factors sequential photo strand opening single molecule repair pathways translocation process answer questions strain d.n.a. incorrect direction strand d.n.a. dna frame rate 1000 nucleotides chalk talk recipient d.n.a. good question molecules target search process good answer caption complete d.n.a. sequence difficult problem microscope slide single strand biologically efficient homology search molecular biology open door fusion based guy d.n.a. biological problem search problem papers including d.n.a. motor proteins bioinformatics ology search genetics donor d.n.a. individual d.n.a. molecule definitive proof correct sequence multiple copies chemistry 1st question search complex single strand d.n.a. catalytic activity 1st time individual molecule recount nation matching copy d.n.a. repair expected target single molecule imaging fold drop longer pieces From Joan Olson on April 7th, 2020 23 plays
34:24 CNF 30th - Nanostructures and X rays for Nano… CNF 30th - Nanostructures and X rays for Nano Characterization	CNF 30th - Nanostructures and X rays for Nano Characterization nano assembler concepts in physics reconstructed image nano science geography electron microscopy heat transfer carbon edge optical devices electronic configurations physical chemistry photography high aspect ratio nanotechnology good efficiency geography terminology diffraction pattern science of photography core level lecture trace element mapping microscopy spatial resolution non-green biomass fertility optics slight aliasing carbon loading carbon absorption double hydroxide molecular orbital infertile males quantum chemistry large diameter chemical properties dimensional focusing atomic physics geochemistry circular structures lawrence berkeley lab focal length aspect ratio structure glass physics imaging ratios zone width trace element photon energy high resolution imaging length phase contrast scientific techniques outermost zone microscopes image processing management prefectures in france ray optics physical optics core level nuclear waste geometrical optics nice point focus rapid progress high resolution absorption edge stony brook refractive focusing maximum angle good material spectroscopy pattern recognition algorithm analytical chemistry huge gain caption complete computer graphics economics terminology biochemistry nano fabrication circles nanometer resolution total internal reflection mediasite limiting factor zone plate avoid proximity black groups 3play_vodcnf_standard From Karlis Musa on February 19th, 2020 6 plays Content Categories Presentations Creation Date June 14th, 2007 Creator 1 First Name Christopher Creator 1 Last Name Jacobson
00:29 David Lynch - NanoNuggets NanoDays 2016 David Lynch - NanoNuggets NanoDays 2016	David Lynch - NanoNuggets NanoDays 2016 improve enable remember display technology c.r.t. circuits resolution industry digital imaging nanotech years stability efficiency display expect 3play_vodcnf_standard c.n.n. panel technology pixel caption complete From David Botsch on January 24th, 2020 1 plays
56:17 Electron Tomography - Practical Aspects Electron Tomography - Practical Aspects	Electron Tomography - Practical Aspects 2017 PARADIM SUMMER SCHOOL Practical Tomography Robert Hovden dimensional space degree tilt hot pixel surface rendering low tilt projection requirement chemical elements slab geometry tilt series symbols of utah transparency curve medium filter vertical direction image shift reconstruction quality operation size signal processing small gold high quality reconstruction uncorrected system center mass 1st thing convergence angle electronics symbols single projection single axis original object telegraphic reconstruction real space high values hand side post processing tomography holder stem tomography s.d.o. high tilt slight crescent moon 180 degree typical data set cross correlation high intensity crescent moon shape biology and pharmacology of chemical elements noise filtering segmentation candy wrapper bounding box nano particles dynamic range graphic reconstruction medical imaging intensity values tilt axis cubic minerals contour surface linear drift higher resolution tilt angle assess reconstruction good alignment center ground natural feel morphological operation manual alignment higher intensity copper bars demographic reconstruction length alignment procedure focal plane $180.00 degrees 3d computer graphics bilinear operators nano cube data set carbon support aberration corrected tool defined peaks center particle peek representing politics dynamic focusing caption complete reconstruction post processing color map fit lots center object directional case higher tilt peter talked tilt range alignment quality gal shambler fully opaque projection image minus $75.00 gold particles low values covariance and correlation traditional tomography regime object size $32.00 bit materials electrical conductors elementary mathematics From James Overhiser on February 15th, 2019 9 plays
01:23:44 Electron Tomography - Theory Electron Tomography - Theory	Electron Tomography - Theory 2017 PARADIM SUMMER SCHOOL Tomography Theory Ercius cartesian space dimensional space dimensional thing scale tomography dimensional reconstruction projection requirement solutions extremely high resolution original image physical science maximum tilting tilt series long time electron microscopy electron tomography compressed sensing inverse nanometers scientific techniques dimensional structure materials science equal angle signal processing concepts in physics photography low dose 1st thing point defect higher frequency single projection diffraction pattern pretty good microscopy needle sample big difference free transform original object spatial frequency vision carbon atom real space adam counting reciprocal space emerging technologies cat f.f.t. dimensional image perfect projections original projection atomic physics projected density experimental projections nano particles stem medical imaging high resolution carbon good reconstruction original projection direction unique object aberration corrected atomic scale beam electrons experimental data tilt axis original intensity nanometer depth pseudo polar microscopes reconstruction technique projection operation low frequency radio spectrum equally slope tomography quantum mechanics kinematics concentric squares cat scan higher dimensional quantum dot analytic geometry iterative loop entire image equal distance iterative algorithm classical mechanics digital photography called equally slope linear algebra grand challenge single particle simpler interpellation dimensional object geometry limitation high tilts nano scale phoria transform english alternative rock groups resolution tomography reconstruction packages building block caption complete digital geometry computer graphics free space reconstruction algorithm improve reconstructions high frequency atomic resolution projection image shell particle cohen resolution dimensional picture sine wave crystallographic defects t.m. advanced algorithms iterative process missing wedge information theory continuous function material science tomography experiment broadcasting mass sample things elementary mathematics chemistry electron microscope nanomaterials From James Overhiser on February 15th, 2019 15 plays
39:59 Introduction to Electron Microscopy Introduction to Electron Microscopy	Introduction to Electron Microscopy 2017 PARADIM Intro to TEM David Muller aperture size observational astronomy data sets big objects scattering individual atoms long time electron microscopy optical devices fraction pattern molecules electromagnetic potential electron microscope big fat spot atomic force microscope strong lens 1000000 volts concepts in physics photography magnetic field c.c.d. camera numerical aperture 1st thing diffraction limited zation damage diffraction pattern huge cross sections charge carriers spherical aberration microscopy big angles optics objective lens chip failed 1st electron bacteria optical microscope radiation damage atomic physics imaging mode uniformly illuminated electron lens electron beam aberration correct magnetism lens action energy loss s.c.m. magnetic lens scientific techniques microscopes optic axis statistics accelerator physics transmission electron microscope geometrical optics science of photography scanning electron microscope incredibly incredibly tiny knowledge representation projectile lenses diffraction spot physical quantities digital photography diffraction blur objective aperture viewing screen magnetostatics condenser lens caption complete free space c.c.d. scattering mechanism particle physics electron gun great amount small spot atomic resolution single transistor time varying field elastic scattering high voltage t.m. electromagnetism charged particles electron military equipment academic administration losses equation From James Overhiser on February 15th, 2019 39 plays
01:01:34 Linear Imaging Theory Linear Imaging Theory	Linear Imaging Theory 2017 PARADIM Linear Imaging Theory Robert Hovden d.n.a. dark field aperture size object function total intensity collaboration increases concepts in physics convergence angle area spherical aberration wide range covering today geometric shapes d.n.a. structure central minimum electron microscopy focal point intensity increases linear imaging model wave vectors control theory optical devices collection angle larger aperture electron microscope high angle architectural elements signal processing photography high pass filter transfer function uncorrected system phase shift darkfield regime functional form scattered electrons higher frequency bright field stem egyptian artefact types diffraction pattern science of photography point spread function high intensity probe elementary geometry integrals spatial frequency optics objective lens bright field regime bright spots real space low frequency reciprocal space blurring contribution sphere collaboration uncorrected instrument characteristic dimension incoherent imaging condition surfaces aperture function wave front atomic physics imaging mode contract transfer function white noise scanning transmission electron cohere imaging previous lecture distant plane elementary shapes stem higher angles high resolution incoherent linear image mil rating detector geometries field cone geometry types of functions aberration corrected constant phase length lower frequencies chromatic aberration conic sections amplitude squared aberration free scientific techniques microscopes measuring instruments intensity squared optic axis intensity decreases contrast transfer diffraction limit stem image individual atom radio spectrum incoherent imaging quantum mechanics aberration free probe multivariable calculus people meters focal plane bright field low angle amateur radio systems theory image plane coherent imaging condition collecting electrons blurring function transmission electron microscopy physical quantities digital photography angular spread double integral coherent imaging largest range face shift higher order aberrations raleigh criterion pio fairly interpretable transmission electron microscope square pulse geometrical optics digital signal processing 1st order caption complete probe tails nyquist limit blue curve subroutines broadcasting filter frequency response maximum frequency high frequency caustic curve hand side low pass filter spatial coordinates atoms closer coherent image sparrow criterion structural system pico meter dark background frequency space optimal aperture t.m. complex conjugate contrast transfer function wave function measure ultimately imaging condition annular darkfield incoherent imaging mode atomic number field stem atomic column inelastic interactions nobel prize angle medium chemistry focal imaging probe size From James Overhiser on February 15th, 2019 31 plays
01:29:35 Intro to EELS (Part 1) Intro to EELS (Part 1)	Intro to EELS (Part 1) 2017 PARADIM Intro to EELS part 1 Paolo Longo c.l. chemical shift concepts in physics inelastic interaction core shell components component reference spectrum criminal law conversion factor ontology (information science) d.s. chemical face chemical analysis nuclear physics electrons coming deal suspect decelerating voltage large area equally scott conduction bend reference scouting events suspect romeo microscopy measurement b.c. integration window coral select relative amount conventional microscope atomic physics scientific terminology pretty good nano particles historical scientific instruments t.v. energy field spectrum integral silicone oxide energy loss geometry elemental mapping e.l.c. logic m.l.s. fine structure eagerly scott spectrum imaging philosophical logic single detector anions eel suspect full spectrum quantum mechanics data management collection angle windows method e.d.s. coral sea region flowers semantics plants physical quantities electromagnetic spectrum pretty edge 2nd blossom spectroscopy l.a. elsa spectrum aluminum alleviate fin specimen protocol session caption complete low energy angle c.c.d. def jam upper level total spectrum entire spectrum optical spectrum scientific classification t.m. bulk silicone good energy energy range reading voltage extended fine structure From James Overhiser on February 15th, 2019 27 plays
$Thumbnail for entry Diffraction (Part 2)$ 01:07:41 Diffraction (Part 2) Diffraction (Part 2)	Diffraction (Part 2) 2017 PARADIM Diffraction part 2 David Muller central beam noise source aperture size n.c. concepts in physics ideal d.q. 2nd thing readout noise high dynamic range mental plane thermal noise long time channel gain variations quantum electrodynamics digital imaging count rate optical devices fraction pattern pico amps long range biological imaging range electric fields huge amount signal processing geology d.p. photography thin pixel magnetic field hole pez c.c.d. camera electronics poisson distribution diffraction pattern electron hope point spread function 200 kilovolts $16.00 pico amps neighboring pixel square root mass signal standard deviation detective quantum efficiency spatial frequency optics real space research methods energy distribution magnetic ripples d.q. single electron sensitivity particle physics narrow pixel space measurement slowly varying abled sphere atomic physics imaging mode electric field energy deposited minus sigma pretty good emerging technologies electron beam noise dynamic range point $16.00 pico magnetism dock noise soft moon k.v. pulse counting detector lastic imaging modes e.q. low energy pretty darn good electrons arrive micron pixel fire electron random noise microscopes perfectly linear discrete distributions electron produces mass image percent variation technical details electron hole pay q.e. statistics electrostatics electronics terminology quantum mechanics field image single actor ideal detector multiple pixels grain contrast systems theory electron hole 1st time high count rates charge carriers huge number diffraction spot physical quantities digital photography full diffraction pattern noise time pulse counting beam energy high voltages output noise milli 2nd dynamical systems output signal low count rate dimensional data digital signal processing momentum transfer caption complete $150.00 micron information pixel array detector c.c.d. 1000 electrons laboratory equipment cybernetics atomic resolution scientific method finite size fundamental limit noise ratio fraction spot quantum chemistry t.m. data evaluation methods electromagnetism momentum operator incident electron diffraction probability current flow rough rule beam current wave function diffraction work From James Overhiser on February 15th, 2019 29 plays
$Thumbnail for entry Diffraction (Part 1)$ 01:18:03 Diffraction (Part 1) Diffraction (Part 1)	Diffraction (Part 1) 2017 PARADIM Diffraction part 1 David Muller d.n.a. solid pole concepts in physics outgoing spherical wave positive nuclei ray diffraction experiment atomic physics diffracted beam nicely lined higher order silicon atom electron microscopy focal point image intensity zone axis symmetry zone axes optical devices fia fraction pattern teaching shape factor electron microscope audio engineering high angle nuclear physics fit scattering beautiful thing good approximation thin sample imaging method chip chip chip reciprocal lattice phase shift elastic scattering digital audio english indie rock groups diffraction pattern diffraction space geometric optics chemical elements big angles spatial resolution small angle scattering elementary special functions badly tilted spatial frequency adam layer real space lattice spacing delta function small angle measurement crystallography particle physics musical quintets time frequencies quantum chemistry scattering happened 1st column scattering oral communication plane wave atom column biology and pharmacology of chemical elements titanium column multi slice transmission function electron beam finite object surface normal big spacing periodic crystal soft moon forward direction synchrotron-related techniques wave number constant phase infinite series change shape travel parallel infinitely large crystal scientific techniques main thing sol microscopes mathematical physics contrast imaging optic axis shape fact transition metals initial wave forward scattering stem image reciprocal space spherical wave electronics terminology quantum mechanics field image neighboring column 1st approximation reducing agents fourier analysis analytic geometry furcal wave focal plane time varying bright field angle scattering order spot multi slice simulation image plane didactics scattering event physical aperture diffraction spot momentum space physical quantities phoria transform times worse 2nd ring single atom objective aperture dietary minerals absorption and radiative transfer (optics) trigonometry strong phase approximation unit cell field imaging carbon nitrogen stem mode metals diffraction peak inverse angstroms english alternative rock groups dog field geometrical optics parallel beam 1st order momentum transfer atomic resolution image year transform crystallography definition periodic table fiber optic waveguide pyrotechnic fuels central spot semiconductor materials mathematical notation caption complete finite size lattice image d.c. large angle fall field sine wave fraction spot functional analysis random orientation t.m. bad news initial wave function educational psychology acoustics information theory diffraction atomic number long rod wave function From James Overhiser on February 15th, 2019 47 plays
55:09 Lee2 Lee2	Lee2 summer natural sciences magnetic observational astronomy chemical elements solutions geometric shapes scattering thermodynamic entropy knowledge continuum mechanics systems theory metallurgy thermodynamics optical devices systems factor transformation physical chemistry nuclear physics pharmacology linear filters concepts in physics differential topology rotation hamiltonian rays scattering neutron related techniques magnet elementary geometry phases of matter magnetic ordering sociology of culture optics force atsic statistical mechanics energy philosophical terminology spin models crystallography particle physics surfaces hexagonal minerals quantum chemistry impurity dietary minerals atomic physics research experiment biology and pharmacology of chemical elements electronics statistical terminology elementary shapes magnetism imaging carbon drug delivery devices topology building materials chemical processes interaction natural resources geometry low energy transfer dimensionless numbers probability theory house types ray techniques considerations symmetry color communication community and uncertainty colloidal chemistry transition metals x-ray instrumentation temperature natural philosophy native element minerals quantum mechanics information science structure pi analytic geometry direction molecular biology volume glass heat transfer biomolecules classical mechanics condensed matter physics meteorology crystal solvents spectroscopy absorption and radiative transfer (optics) hippie movement mechanics aesthetics analytical chemistry metals theoretical physics surveying large intensity education caption complete information nearest neighbor energy transfer web colors darkness scientific method neutron quantum field theory anatomy types of organization liquids velocity covariance and correlation electromagnetism light crystals materials mass correlation electrical conductors resolution chemical engineering From James Overhiser on September 12th, 2018 25 plays
31:32 16 - The Adolescent Brain: New Views from… 16 - The Adolescent Brain: New Views from Neuroimaging	16 - The Adolescent Brain: New Views from Neuroimaging decision behavior attention organs evolution development divorce kinematics optical spectrum youth notion integration concepts in aesthetics mediasite neuroscience evaluation horticulture and gardening risk i.q. brain legal procedure web colors decision theory parts of speech childhood biology educational psychology cerebrum happiness neurons psychiatry magnetic resonance imaging concepts in physics on premise d.n.a. film and video technology m.r.i. plant taxonomy plant morphology medicine medical terminology science neuroanatomy central nervous system organization family connectivity american action thriller films literature technologies biological concepts economics terminology neuroscientist mechanics maturation management women constitutional law psychology autonomy htl2 color early species of homo human cutting chemistry difference adolescent tool-using species death connections creativity terms experimental psychology destination networks motivation subjects taught in medical school personal life human behavior evolutionary biology caption complete year olds molecules thing adolescence disasters problem solving planning biology terminology genetics reproduction environment unsolved problems in neuroscience emotion cit mediasite recordings physical objects sensory system legal terms developmental psychology graph theory matter attention-deficit hyperactivity disorder elections From Marshall Perryman on May 19th, 2018 8 plays
34:18 06 - Risk and Ambiguity in the Nervous System 06 - Risk and Ambiguity in the Nervous System	06 - Risk and Ambiguity in the Nervous System astronomical imaging social sciences systems science risk limbic system behavioral economics risk averse behavioral concepts underlying principles of microeconomic behavior financial economics finance currency optical devices boolean algebra financial risk geography mediasite virtue business neuroscience theories of mind political economy demography economic anthropology social philosophy design of experiments behavioral finance cognitive science brain point web colors proof theory decision theory intelligence data management philosophy central nervous system international trade ontology commerce concepts in physics on premise representation subjective ventral striatum interest money macroeconomics game theory actuarial science risk attitude brain areas choice consumer theory economics of uncertainty paradoxes stock market renting human geography human rights lottery reasoning international economics mathematical logic basal ganglia contemporary philosophy utility human behavior risk neutrality learning accountancy economic systems management marketing scientific method semantics psychology autonomy e.u. htl2 color social psychology food and drink standard theories philosophical logic imagination risk neutral probability theory differential calculus business law metalogic experiment theory of probability distributions creativity representative property pedagogy risk aversion attitude nutrition conic sections probability personhood microeconomics functions and mappings metaphysics of mind evaluation methods philosophical terminology foreign exchange market topology international organizations of europe caption complete statistical terminology value theory thing economic theories prospect theory subject financial markets function interesting truth differential geometry payment systems philosophy of psychology experiments economist unsolved problems in neuroscience emotion cit mediasite recordings logic investment optical spectrum islam developmental psychology From Marshall Perryman on May 19th, 2018 10 plays
55:40 CAM Colloquium September 19, 2014 - Julianne… CAM Colloquium September 19, 2014 - Julianne Chung: Designing Optimal Spectral Filters and Low-Rank Matrices for Inverse Problems	CAM Colloquium September 19, 2014 - Julianne Chung: Designing Optimal Spectral Filters and Low-Rank Matrices for Inverse Problems Computing reliable solutions to inverse problems is important in many applications such as biomedical imaging, computer graphics, and security. Regularization by incorporating prior knowledge is… web colors concepts in physics developmental psychology environmental science cognitive science theoretical computer science parker brothers games risk geography formal sciences general case engineering mathematical optimization determinants matrix theory materials systems science accountancy papermaking market research factorial and binomial topics scale problems digital signal processing data analysis evaluation methods statistics noise applied mathematics design of experiments probability theory statistical charts and diagrams computer graphics international law regression analysis theory of probability distributions family mathematical analysis mediasite systems financial economics hematology natural philosophy tissues gamma and related functions idealism sociology index psychology filter summary statistics manufacturing structural system information science research problem robust statistics differential calculus operator theory mathematical logic thing binary operations sensory system computer programming abstract strategy games social groups filter factor functions and mappings c.s.v. digital imaging noise level time series analysis fall 2014 injuries creativity research methods historical eras estimation theory solution exponential family distributions cybernetics scientific method image processing parameter mathematical notation mathematical objects optimal filter graphic design mathematical and quantitative methods (economics) classical mechanics caption complete learning on premise linear operators in calculus functional analysis inverse problems eng digital photography t.s.p. group theory image geography terminology logic factor low rank linear algebra truth color air filter philosophical terminology base risk investment normal distribution political philosophy pedagogy physiology good reconstruction signal processing data systems theory error legal doctrines and principles photography statistical terminology compute optimal quality training data covariance and correlation deduction board games input/output reconstruction statistical inference step process polynomials information educational psychology evaluation communication inverse problem statistical theory cartography From E. Cornelius on May 4th, 2018 5 plays
57:56 CAM Colloquium, 2014-01-31 - Guillaume Bal:… CAM Colloquium, 2014-01-31 - Guillaume Bal: High-contrast High-resolution Coupled Physics Imaging Modalities	CAM Colloquium, 2014-01-31 - Guillaume Bal: High-contrast High-resolution Coupled Physics Imaging Modalities ultrasound materials engineering radioactivity cam specification languages sound computer systems electromagnetic radiation symbols physical sciences systems science guillaume bal cornell p.d. mathematical objects natural sciences greek alphabet color interaction equations of physics vector calculus genetics image processing equation problem acoustics function communication control theory scientific method physical objects nuclear physics photometry caption complete idealism mechanics quantum electrodynamics sheer waste economic anthropology send light tissue information science metals imagination systems set theory seafood latin alphabet wireless linear operators in calculus evaluation personal life information theory statistical theory group theory logical consequence reasoning linear algebra mental structures religion polynomials forestry light statistical terminology interest hearing computing terminology u.t. m.r.i. high contrast medical terminology nuclear magnetic resonance meteorology light cone r.p.g elementary algebra natural resources measurement medical imaging organs mass determinants reconstruction photography banking partial differential equations condition structural system broadcasting health electronics occupational safety and health property computer programming algebra creativity computer graphics architectural elements optics particle physics knowledge representation low contrast reconstruct high resolution differential topology medical physics grammar inverse problem contrast vision food ingredients physics anatomy photon syntactic entities european space agency philosophy disambiguation continuum mechanics electromagnetism radio spectrum atomic physics universe value papermaking matter applied mathematics physical cosmology artificial satellites orbiting earth systems theory mathematical analysis force resolution business law accountancy scattering natural philosophy infinity applied and interdisciplinary physics existentialist concepts mechanical vibrations question aesthetics classical mechanics From E. Cornelius on June 29th, 2017 1 plays
55:40 CAM Colloquium, 2014-09-19 - Julianne Chung:… CAM Colloquium, 2014-09-19 - Julianne Chung: Designing Optimal Spectral Filters and Low-rank Matrices for Inverse Problems	CAM Colloquium, 2014-09-19 - Julianne Chung: Designing Optimal Spectral Filters and Low-rank Matrices for Inverse Problems information theory systems science input/output audio engineering sequenced genomes environmental science educational psychology mathematical objects algebra market research signal processing machine learning training data digital photography normal distribution low rank applied mathematics thing graphic design parameter information probability distributions risk minimisation psychotherapy filter factor spacecraft launched by delta ii rockets video psychology time series analysis norm case probability theory artificial intelligence caption complete evaluation social psychology creativity linear algebra real life mathematical optimization noise sample forward model mathematical logic financial economics filter binary operations information science statistical theory regression analysis summary statistics t.s.p. cornell communication cam metrology matrices concepts in physics quality statistical inference decision theory family data management pedagogy web colors air filter natural philosophy group theory functions and mappings intelligence mathematical notation formal sciences photography cybernetics classical mechanics error numerical analysis statistics computer graphic artifacts cognitive science research reconstruction air image mathematical analysis optimal spectral psychometrics logic international law demography neural networks color determinants digital signal processing major cities functional analysis noise scientific method covariance and correlation differential calculus optimal filters robust statistics inverse problem image research methods statistical charts and diagrams measurement polynomials image processing sociology index theoretical computer science design of experiments inverse problems exponential family distributions home automation theory of probability distributions elementary algebra noise level semantics manufacturing data julianne chung estimation theory computer programming computer graphics digital imaging cartography engineering sensory system statistical terminology data analysis systems theory learning philosophical terminology systems problem evaluation methods geography linear operators in calculus From E. Cornelius on June 28th, 2017 27 plays